coronavirus 229e atcc vr 740 Search Results


hcov 229e  (ATCC)
96
ATCC hcov 229e
Divergent activity in downregulating cGAS and STING among human CoV Nsp15. ( a, b ) HEK293T cells were transfected with cGAS or STING plasmid plus mCherry or HCoV Nsp15 plasmids for 48 h. Western blot performed to assess the expression of indicated proteins. ( c ) Protein analysis of the HEK293T cells transfected with cGAS or STING plasmid along with mCherry, SARS-CoV-2 <t>and</t> <t>HCoV-229E</t> Nsp15 (WT and corresponding EndoU mutant) plasmids for 48 h.
Hcov 229e, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/coronavirus+229e+atcc+vr+740/pmc13016584-45-21-22?v=ATCC
Average 96 stars, based on 1 article reviews
hcov 229e - by Bioz Stars, 2026-07
96/100 stars
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atcc vr740 282  (ATCC)
96
ATCC atcc vr740 282
Divergent activity in downregulating cGAS and STING among human CoV Nsp15. ( a, b ) HEK293T cells were transfected with cGAS or STING plasmid plus mCherry or HCoV Nsp15 plasmids for 48 h. Western blot performed to assess the expression of indicated proteins. ( c ) Protein analysis of the HEK293T cells transfected with cGAS or STING plasmid along with mCherry, SARS-CoV-2 <t>and</t> <t>HCoV-229E</t> Nsp15 (WT and corresponding EndoU mutant) plasmids for 48 h.
Atcc Vr740 282, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/coronavirus+229e+atcc+vr+740/pm22673931-125-27-27?v=ATCC
Average 96 stars, based on 1 article reviews
atcc vr740 282 - by Bioz Stars, 2026-07
96/100 stars
  Buy from Supplier

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Divergent activity in downregulating cGAS and STING among human CoV Nsp15. ( a, b ) HEK293T cells were transfected with cGAS or STING plasmid plus mCherry or HCoV Nsp15 plasmids for 48 h. Western blot performed to assess the expression of indicated proteins. ( c ) Protein analysis of the HEK293T cells transfected with cGAS or STING plasmid along with mCherry, SARS-CoV-2 and HCoV-229E Nsp15 (WT and corresponding EndoU mutant) plasmids for 48 h.

Journal: The Journal of General Virology

Article Title: SARS-CoV-2 Nsp15 facilitates immune evasion and viral replication by limiting multiple host innate immune pathways, including cGAS–STING

doi: 10.1099/jgv.0.002233

Figure Lengend Snippet: Divergent activity in downregulating cGAS and STING among human CoV Nsp15. ( a, b ) HEK293T cells were transfected with cGAS or STING plasmid plus mCherry or HCoV Nsp15 plasmids for 48 h. Western blot performed to assess the expression of indicated proteins. ( c ) Protein analysis of the HEK293T cells transfected with cGAS or STING plasmid along with mCherry, SARS-CoV-2 and HCoV-229E Nsp15 (WT and corresponding EndoU mutant) plasmids for 48 h.

Article Snippet: Codon-optimized ORFs of Nsp15 from SARS-CoV-2 (Wuhan-Hu-1 strain, GenBank: NC045512), SARS-CoV (Urbani strain, GenBank: AY278741 ), MERS-CoV (EMC/2012 strain, GenBank: NC019843), HCoV-229E (ATCC VR-740, GenBank: AF304460 ), HCoV-OC43 (ATCC VR-759, GenBank: AY585228 ), HCoV-HKU1 (GenBank: NC006577) and HCoV-NL63 (Amsterdam 1 strain, GenBank: NC005831) fused with C-terminal 1× FLAG or 3× FLAG were inserted into the pcDNA5/FRT/TO vector cut by HindIII (New England Biolabs) and XhoI (New England Biolabs) through In-Fusion cloning (Takara Bio).

Techniques: Activity Assay, Transfection, Plasmid Preparation, Western Blot, Expressing, Mutagenesis